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KMID : 0617319950050010084
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Journal of Pharmacetical Sceiences Ewha Womans University
1995 Volume.5 No. 1 p.84 ~ p.89
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Antitumor Responses of Adoptively-Transferred Tumor-Specific T-Cell Cultures in a Murine Lymphoma Model
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Kim, Hee-Sue
Lee, Hee Gu/Lim, Jong-Seok/Lee, Ki-Young/Kim, Jaewha/Chung, Kyeong-Soo/Choe, Yong-Kyung
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Abstract
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The purpose of this study was to establish an in vitro culture method of tumor-specific T cells, and determine the efficacy of the cultured tumor-specific cytotoxic T-1ymphocytes (CTL) as an agent of anti-tumor immunotherapy against a murine lymphoma, TIMI.4. Tumor-specific T-lymphocytes derived from C57BL/6 mice (thy-1.2) immune to TIMI.4 were activated by in vitro stimulation with the irradiated TIMI.4 cells, and expanded by restimulation with TIMI.4 in the presence of the concanavalin A-stimulated rat spleen culture supematant, and splenic antigen-presenting cells. In vitro restimulation enhanced markedly the proportion of CD8^+, a predominant surface marker of CTL, and the cytotoxic activity in the cultured immune T cell population. The resulting TIMl.4-specific T cells were adoptively transferred into nude mice. The tumor cells residing in the host after 7 days of adoptive transfer to B6.PL (thy-1.1) mice were quantified by use of an antibody directed to the thy-1.2 allele. The TIMI.4 cells in the recipient nude mice were decreased in a dose-dependent manner. Anti-tumor activity of the TIMI.4-specific T cells was also demonstrated by a survival test, where the tumor-bearing nu/nu mice which received the activated T-cells survived about 30% longer than the control mice which received the tumor cells alone. These suggest that adoptive transfer of TIMI.4-specific T cells could be a candidate for effective therapy of the murine lymphoma.
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